Applications

·         Preparation of DNA from Gram-positive and Gram-negative bacteria.

·         DNA can be used for PCR, restriction digests, pulsed-field gel electrophoresis (PFGE), and optical mapping.

The QuickExtract™ Bacterial DNA Extraction Kit provides a simple method for extracting DNA from Gram-positive and Gram-negative bacteria. To a washed pellet of the bacterial sample, add QuickExtract Bacterial DNA Extraction Solution and Ready-Lyse™ Lysozyme Solution. Ready-Lyse Lysozyme is a proven reagent with over 200 times the specific activity of egg-white lysozyme. Mix and incubate for 15 minutes at room temperature. For some species, if lysis is not achieved, the incubation time can be extended. Samples may optionally be heated at 80°C for 2 minutes to kill any remaining viable bacteria. The DNA is now ready for PCR or other downstream applications such as restriction digests, PFGE (pulsed-field gel electrophoresis), and optical mapping. The kit has been tested on a range of bacteria (Table 1) and has been shown to produce very long DNA strands (Fig. 1).

Benefits

·         Single-tube system.

·         No toxic organic solvents.

·         Suitable for high-throughput applications.

·         Ready-Lyse™ Lysozyme supplied.

·         Long DNA generated.

Table 1. Species of bacteria that have been tested with the QuickExtract™ Bacterial DNA Extraction Kit.

Figure 1. Long DNA strands from E. coli extracted using the QuickExtract™ Bacterial DNA Extraction Kit. Many strands are 400-500 kb. Image courtesy of Dr. Shiguo Zhou, University of Wisconsin-Madison.